HYPURE™ OIL: HEAVY / LIGHT
Pre-washed paraffin oils used as protective barrier to prevent the quick evaporation of the medium and to keep specimens safe from external contaminants during in-vitro culture.
Hypure™ Oils play an important role in maintain the stability of the embryo culture system in terms of temperature, pH and osmolarity.
Kitazato Hypure™ oil is a high quality paraffin oil. It is pre-washed with ultra-pure water to reduce toxicity and remove endotoxins, therefore improving your clinical outcomes. Furthermore, washing and saturating oils with ultra-pure water avoids critical components of the media getting absorbed into the oil, which could affect the concentration of the media. That’s the importance of using a Hy-Purity Oil.
Main characteristics
• Pharmaceutical grade quality paraffin oil
• Allows small volumes of medium to be used as it prevents the evaporation of media
• Maintains stable osmolality and pH in culture medium
• Available in glass or PETG bottles
• Hypure Oil™ Heavy is pre-washed and offers extensive protection and insulation
• Hypure Oil™ Light is double-washed and allows easy pipetting
PARAFFIN OR MINERAL OIL?
Mineral and paraffin oils are commonly used to overlay IVF media to maintain their stability throughout embryo culture, but their quality can vary greatly.
Paraffin oil is a specific type of mineral oil that is known to be less sensitive to photooxidation and peroxidation.
High levels of peroxides in oils may have a detrimental effect on fertilization and embryo development, so the choice of culture oil is extremely important as it has a direct effect on clinical outcomes.
Kitazato Hypure™ oil is a high quality paraffin oil. It is pre-washed with ultra-pure water to reduce toxicity and remove endotoxins, therefore improving your clinical outcomes.
Furthermore, washing and saturating oils with ultra-pure water avoids critical components of the media getting absorbed into the oil, which could affect the concentration of the media.
That’s the importance of using a Hy-Purity Oil!
Hypure™ Oils are available with different density and viscosity to better suit laboratory needs. Since both are highly efficient in maintaining media stability, each laboratory could be able to make its own choice of which oil to use for each procedure. Hypure™ Oil Heavy is the perfect choice for processes that require an oil overlay that allows easy handling with a reduced risk of spills or droplet mixing.
Due to its density and viscosity Hypure™ Oil Heavy provides extensive protection and insulation for the culture and is highly efficient in maintaining stable conditions in terms of temperature, pH and osmolality, especially during an extended culture or when using time lapse incubators.
THE BEST OPTION FOR CULTURE IN DRY INCUBATOR
A recent study compared the performance of our Hypure™ Oil Heavy versus similar culture oils from our competitors. This study evaluates its manipulation, physico-chemical properties, and its effects on the stabilization of culture conditions and embryo development.
They have concluded that:
• Helps to maintain a stable pH for a longer period than other oils.
• Has the highest capacity to prevent evaporation when cultured in dry incubator
• Reaches the lowest osmolality after seven days of culture.
• Provides notably better embryo development and cell count rates when compared with these competitors.
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